Human Flt-3 Ligand Quantikine ELISA Kit

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Human Flt-3 Ligand Quantikine ELISA Kit Summary

Human Flt-3 Ligand Quantikine ELISA Kit Summary

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货号：DFK00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL)

Sensitivity

7 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human Flt-3 Ligand

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Flt-3/Flk-2 Ligand Immunoassay is a 4.0 or 4.5 hour solid phase ELISA designed to measure soluble human Flt-3 Ligand in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Flt-3 Ligand and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant Flt-3 Ligand accurately. Results obtained measuring natural human Flt-3 Ligand showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Flt-3 Ligand.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	65.7	214	421	72.8	224	429
Standard Deviation	1.2	4.1	9.3	6.7	13.8	30.5
CV%	1.8	1.9	2.2	9.2	6.2	7.1

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	69.5	230	453	78.2	241	462
Standard Deviation	1.9	5.2	6.2	8.7	14.9	33.1
CV%	2.7	2.3	1.4	11.1	6.2	7.2

Recovery

The recovery of Flt-3 Ligand spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	102	92-118
Citrate Plasma (n=5)	101	94-110
EDTA Plasma (n=5)	101	90-119
Heparin Plasma (n=5)	100	88-112
Serum (n=5)	100	91-117

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of Flt-3 Ligand in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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