Human GM-CSF Quantikine ELISA Kit

返回产品中心

Human GM-CSF Quantikine ELISA Kit

Human GM-CSF Quantikine ELISA Kit Summary

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

货号：DGM00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)

Sensitivity

3 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human GM-CSF

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human GM-CSF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure GM-CSF levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human GM-CSF and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human GM-CSF accurately. Results obtained using natural human GM-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural GM-CSF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	54.7	156	321	53.1	157	317
Standard Deviation	1.1	3.2	4.8	3.3	9.1	15.4
CV%	2	2.1	1.5	6.2	5.8	4.9

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	46.8	131	269	45.1	133	266
Standard Deviation	1.1	3.6	7.3	2.4	7	11.5
CV%	2.4	2.7	2.7	5.3	5.3	4.3

Recovery

The recovery of GM-CSF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	97	85-104
Citrate Plasma (n=5)	101	92-106
EDTA Plasma (n=5)	100	94-105
Heparin Plasma (n=5)	98	92-104
Serum (n=5)	98	93-103

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of GM-CSF in various matrices and diluted with the appropriate Calibrator Diluent to produce values within the dynamic range of the assay.

Human Flt-3 Ligand Quantikine ELISA Kit Summary

Porcine TNF-alpha Quantikine ELISA Kit Summary

返回 产品中心