Human Galectin-3 Quantikine ELISA Kit Su

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Human Galectin-3 Quantikine ELISA Kit Summary

Human Galectin-3 Quantikine ELISA Kit Summary

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货号：DGAL30

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

0.085 ng/mL

Assay Range

0.3 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Galectin-3. This assay cross-reacts 58% with Mouse Galectin-3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Galectin-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Galectin-3 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Galectin-3 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Galectin-3 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Galectin-3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	0.79	2.46	5.11	0.8	2.59	5.5
Standard Deviation	0.03	0.09	0.18	0.05	0.16	0.32
CV%	3.8	3.7	3.5	6.3	6.2	5.8

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	0.78	2.27	5.06	0.81	2.51	5.29
Standard Deviation	0.03	0.1	0.15	0.07	0.2	0.36
CV%	3.8	4.4	3	8.6	8	6.8

Recovery

The recovery of Galectin-3 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	96-106
EDTA Plasma (n=4)	105	93-113
Heparin Plasma (n=4)	103	95-110
Serum (n=4)	104	94-115

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of human Galectin-3 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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