Human Serpin E1/PAI-1 Quantikine ELISA K

Human Serpin E1/PAI-1 Quantikine ELISA Kit Summary

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货号：DSE100

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Cell Lysates (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL), CTAD Plasma (50 uL)

Sensitivity

0.142 ng/mL

Assay Range

0.3 - 20 ng/mL (Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, CTAD Plasma)

Specificity

Natural and recombinant human Serpin E1

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human Serpin E1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Serpin E1 in cell culture supernates, cell lysates, and plasma. It contains Sf 21-expressed recombinant human Serpin E1 and has been shown to accurately quantitate the recombinant factor in its active and latent forms as well as in the vitronectin complexes. However, the kit does not detect recombinant human Serpin E1 in complexes with recombinant human uPA and recombinant human tPA. Results obtained using natural human Serpin E1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin E1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, CTAD Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	2.6	6.5	12.6	2.3	6.4	13.1
Standard Deviation	0.12	0.52	0.98	0.2	0.4	0.8
CV%	4.4	8	7.8	9.5	6.4	6.1

Recovery

The recovery of Serpin E1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	106	89-113
Citrate Plasma (n=4)	99	88-112
CTAD Plasma (n=4)	100	83-112
EDTA Plasma (n=4)	100	85-115
Heparin Plasma (n=4)	98	85-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Serpin E1 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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