Human IL-4 Quantikine ELISA Kit Summary

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Human IL-4 Quantikine ELISA Kit Summary

Human IL-4 Quantikine ELISA Kit Summary

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货号：D4050

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL)

Sensitivity

10 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human IL-4

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-4 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant human IL-4 accurately. Results obtained using natural human IL-4 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-4.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	159	538	1092	169	503	1032
Standard Deviation	11.4	27.8	67.2	16.8	49.3	83
CV%	7.2	5.2	6.2	9.9	9.8	8

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	170	510	1110	132	411	865
Standard Deviation	6.7	17.5	58.5	9.8	32.1	52.8
CV%	3.9	3.4	5.3	7.4	7.8	6.1

Recovery

The recovery of human IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=8)	98	90-109
Citrate Plasma (n=10)	94	85-109
EDTA Plasma (n=10)	100	90-123
Heparin Plasma (n=10)	95	84-121
Serum (n=10)	98	82-111

Linearity

To assess the linearity of the assay, the following biological samples, spiked with high concentrations of IL-4, were diluted with the appropriate Calibrator Diluent and then assayed.

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