Human RAGE Quantikine ELISA Kit Summary

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Human RAGE Quantikine ELISA Kit Summary

Human RAGE Quantikine ELISA Kit Summary

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货号：DRG00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

16.14 pg/mL

Assay Range

78.0 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human RAGE

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human RAGE Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human RAGE (extracellular domain) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human RAGE/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human RAGE showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring RAGE.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	546	1527	3117	519	1449	2890
Standard Deviation	34	73	189	43	119	192
CV%	6.2	4.8	6.1	8.2	8.2	6.7

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	571	1549	3189	563	1568	3159
Standard Deviation	34	73	191	49	110	193
CV%	5.9	4.7	6	8.7	7	6.1

Recovery

The recovery of RAGE spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Tissue Culture Media (n=4)	98	86-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RAGE were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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