Human Chitinase 3-like 1 Quantikine ELIS

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Human Chitinase 3-like 1 Quantikine ELISA Kit

Human Chitinase 3-like 1 Quantikine ELISA Kit Summary

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货号:DC3L10

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)

Sensitivity

8.15 pg/mL

Assay Range

62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human CHI3L1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human CHI3L1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure CHI3L1 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CHI3L1 and antibodies raised against recombinant human CHI3L1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural CHI3L1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human CHI3L1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	566	1031	2202	606	1019	2212
Standard Deviation	26.7	44.4	102.6	32.1	59.1	153.1
CV%	4.7	4.3	4.7	5.3	5.8	6.9

Recovery

The recovery of CHI3L1 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	97	93-100
Urine (n=4)	105	90-115

Linearity

To assess the linearity of the assay, samples containing high concentrations of CHI3L1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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