Human IL-7 Quantikine HS ELISA Kit

返回产品中心

Human IL-7 Quantikine HS ELISA Kit

Human IL-7 Quantikine HS ELISA Kit Summary

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

货号：HS750

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

18-24 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Citrate Plasma (200 uL)

Sensitivity

0.1 pg/mL

Assay Range

0.2 - 10 pg/mL (Cell Culture Supernates), 0.3 - 16 pg/mL (Serum, EDTA Plasma, Citrate Plasma)

Specificity

Natural and recombinant human IL-7

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine HS Human IL-7 Immunoassay is a solid phase ELISA that specifically measures IL-7 in 18-24 hours (with an overnight incubation step). This kit is designed to measure IL-7 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-7 and antibodies raised against the recombinant factor. It has been shown to quantitate the recombinant factor accurately. Results obtained using natural IL-7 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-7.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	0.46	5.79	11.18	0.51	6.38	12.95
Standard Deviation	0.03	0.19	0.44	0.06	0.5	1.3
CV%	6.5	3.3	3.9	11.8	7.8	10

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	0.5	1.49	6.86	0.78	2.13	8.81
Standard Deviation	0.04	0.14	0.61	0.08	0.16	0.64
CV%	8	9.4	8.9	10.3	7.5	7.3

Recovery

The recovery of IL-7 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	103	89-113
Citrate Plasma (n=5)	100	92-110
EDTA Plasma (n=5)	112	83-132
Serum (n=5)	100	91-113

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of IL-7 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Chitinase 3-like 1 Quantikine ELISA Kit

Human IL-6 R alpha Quantikine ELISA Kit Summary

返回 产品中心