The Quantikine Porcine IL-6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant porcine IL-6 and antibodies raised against the recombinant factor. The immunoassay has been shown to accurately quantitate recombinant porcine IL-6. Results obtained using natural porcine IL-6 show dose response curves that are parallel to the standard curves obtained using recombinant Quantikine Porcine IL-6 kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-6.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

The recovery of porcine IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.

To assess the linearity of the assay, samples spiked with high concentrations of porcine IL-6 in each matrix were diluted with Calibrator Diluent and then assayed.