Human CCL20/MIP-3 alpha Quantikine ELISA

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Human CCL20/MIP-3 alpha Quantikine ELISA Kit

Human CCL20/MIP-3 alpha Quantikine ELISA Kit Summary

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货号：DM3A00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)

Sensitivity

0.87 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human MIP-3 alpha

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MIP-3 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure MIP-3 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-3 alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-3 alpha showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIP-3 alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	66.2	180	372	68.7	186	357
Standard Deviation	1.84	3.83	15.1	4.51	13	28.8
CV%	2.8	2.1	4.1	6.6	6.9	8.1

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	55.9	161	323	72.5	200	386
Standard Deviation	1.62	4.28	9.1	6.05	17.9	35.3
CV%	2.9	2.7	2.8	8.3	8.9	9.1

Recovery

The recovery of MIP-3 alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=8)	101	88-115
EDTA Plasma (n=5)	104	97-115
Heparin Plasma (n=5)	104	98-112
Serum (n=5)	103	94-112

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of MIP-3 alpha in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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