Human sE-Selectin/CD62E Quantikine ELISA Kit

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Human sE-Selectin/CD62E Quantikine ELISA Kit

Human sE-Selectin/CD62E Quantikine ELISA Kit Summary

Catalog Availability Size / Price

DSLE00 1 Kit / Please Inquire
SSLE00 1 Pack (6 Plates) / Please Inquire

PDSLE00 1 Pack (50 Plates) / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)

Sensitivity

0.027 ng/mL

Assay Range

0.1 - 8 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human sE-Selectin

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human sE-Selectin immunoassay is a 4.5 hour solid phase ELISA designed to measure sE-Selectin in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human sE-Selectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sE-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human sE-Selectin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	1.6	2.9	4.7	1.5	2.9	4.6
Standard Deviation	0.11	0.17	0.24	0.11	0.25	0.35
CV%	6.6	5.7	5.2	7.3	8.7	7.7

Recovery

The recovery of sE-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	103	98-110
Citrate Plasma (n=4)	96	85-107
Heparin Plasma (n=4)	94	85-107
Serum (n=4)	96	85-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of sE-Selectin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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