Mouse IL-2 Quantikine ELISA Kit

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Mouse IL-2 Quantikine ELISA Kit

Mouse IL-2 Quantikine ELISA Kit Summary

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Catalog Availability Size
M2000 1 Kit (2 Plates)
SM2000 1 Pack (6 Plates)
PM2000 1 Pack (50 Plates)

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL)

Sensitivity

3 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum)

Specificity

Natural and recombinant mouse IL-2

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-2 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-2 accurately. Results obtained using natural mouse IL-2 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	70.4	187	595	70.9	175	559
Standard Deviation	3.9	10	23.4	3.6	9.6	24.5
CV%	5.5	5.3	3.9	5.1	5.5	4.4

Recovery

The recovery of mouse IL-2 spiked to three levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	102	84-110
Serum (n=6)	102	96-108

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-2 in each matrix were diluted with Calibrator Diluent and then assayed.

Mouse IL-2 ELISA Linearity

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