Mouse CCL11/Eotaxin Quantikine ELISA Kit

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Mouse CCL11/Eotaxin Quantikine ELISA Kit

Mouse CCL11/Eotaxin Quantikine ELISA Kit Summary

货号：MME00

规格：96T

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Citrate Plasma (13 uL)

Sensitivity

3 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)

Specificity

Natural and recombinant mouse Eotaxin. This assay cross-reacts 0.062% with Human Eotaxin and recognizes Rat Eotaxin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse Eotaxin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Eotaxin in cell culture supernates, serum, and plasma. It contains recombinant mouse Eotaxin and antibodies raised against the recombinant protein. This immunoassay has been shown to quantitate the recombinant protein accurately. Results obtained using natural mouse Eotaxin showed dose curves that were parallel to the standard curves obtained using the E. coli-expressed kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Eotaxin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	49	197	466	46	179	446
Standard Deviation	3.2	7.2	30	3.8	11.7	28.7
CV%	6.5	3.7	6.4	8.3	6.5	6.4

Recovery

The recovery of mouse Eotaxin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	96	82-103
Citrate Plasma (n=5)	97	81-114
EDTA Plasma (n=5)	100	83-118
Serum (n=5)	98	82-111

Linearity

Human CD40 Ligand/TNFSF5 Quantikine ELISA Kit

Human Myeloperoxidase Quantikine ELISA Kit

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Product Summary

Precision

Recovery

Linearity

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