Human Myeloperoxidase Quantikine ELISA K

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Human Myeloperoxidase Quantikine ELISA Kit

Human Myeloperoxidase Quantikine ELISA Kit Summary

货号：DMYE00B

规格：96T

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Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL), Saliva (10 uL), Urine (50 uL)

Sensitivity

0.062 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Urine)

Specificity

Natural human MPO

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MPO Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human MPO in cell culture supernates, cell lysates, serum, platelet-poor plasma, saliva, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Cell Lysates, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	0.994	3.36	6.68	0.974	2.99	6.23
Standard Deviation	0.034	0.085	0.123	0.078	0.18	0.38
CV%	3.4	2.5	1.8	8	6	6

Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	1.03	3.13	6.63	1.07	3.29	6.71
Standard Deviation	0.024	0.046	0.172	0.12	0.26	0.55
CV%	2.3	1.5	2.6	10.8	8	8.2

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