Mouse Total MMP-9 Quantikine ELISA Kit S

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Mouse Total MMP-9 Quantikine ELISA Kit Summary

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Mouse Total MMP-9 Quantikine ELISA Kit Summary

货号：MMPT90 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.014 ng/mL

Assay Range

0.1 - 5 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)

Specificity

Natural and recombinant mouse MMP-9 (pro-, active, and TIMP-complexed)

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse MMP-9 (total) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total mouse MMP-9 (pro-, active, and TIMP-complexed MMP-9) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse MMP-9 and antibodies raised against the recombinant factor. Natural mouse MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural mouse MMP-9.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	0.239	0.731	1.71	0.211	0.6	1.61
Standard Deviation	0.024	0.037	0.097	0.02	0.049	0.125
CV%	8.4	5.1	5.7	9.6	8.1	7.8

Recovery

The recovery of mouse MMP-9 spiked to 3 levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	94	86-108

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse MMP-9 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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