Mouse Osteoprotegerin/TNFRSF11B Quantiki

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Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit

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Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit Summary

货号：MOP00

规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL)

Sensitivity

6.9 pg/mL

Assay Range

31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)

Specificity

Natural and recombinant mouse OPG

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse OPG Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse OPG in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse OPG and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse OPG accurately. Results obtained using natural mouse OPG showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse OPG.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	69.9	311	650	68	332	741
Standard Deviation	5.5	13.4	35.6	5	23	54
CV%	7.9	4.3	5.5	7.4	6.9	7.3

Recovery

The recovery of mouse OPG spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=9)	101	85-116
EDTA Plasma (n=9)	97	88-107
Serum (n=11)	93	83-108

Linearity

To assess the linearity of the assay, five or more samples containing various concentrations of OPG in each matrix were diluted with Calibrator Diluent and then assayed.

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