Human IGFBP-3 Quantikine ELISA Kit

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Human IGFBP-3 Quantikine ELISA Kit

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Human IGFBP-3 Quantikine ELISA Kit Summary

货号：DGB300 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.14 ng/mL

Assay Range

0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IGFBP-3

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IGFBP-3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IGFBP-3 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human IGFBP-3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGFBP-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGFBP-3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	4.13	12.7	31.1	4.06	11.8	28.5
Standard Deviation	0.2	0.64	0.71	0.22	0.76	2.27
CV%	4.8	5	2.3	5.4	6.4	8

Recovery

The recovery of IGFBP-3 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	94	89-99

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGFBP-3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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