Mouse CCL17/TARC Quantikine ELISA Kit

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Mouse CCL17/TARC Quantikine ELISA Kit

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Mouse CCL17/TARC Quantikine ELISA Kit Summary

货号：MCC170 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL)

Sensitivity

5 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)

Specificity

Natural and recombinant mouse TARC

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse TARC Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse TARC levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse TARC and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse TARC showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TARC.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	27	89	278	29	98	310
Standard Deviation	1.3	2.3	7.8	2.2	6.9	17.3
CV%	4.8	2.6	2.8	7.6	7	5.6

Recovery

The recovery of mouse TARC spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	94	85-113
EDTA Plasma (n=6)	94	83-109
Serum (n=6)	94	82-103

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse TARC in each matrix were diluted with Calibrator Diluent and assayed.

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