Human Vitamin D BP Quantikine ELISA Kit

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Human Vitamin D BP Quantikine ELISA Kit

Human Vitamin D BP Quantikine ELISA Kit Summary

货号：DVDBP0B

规格：96T

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Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)

Sensitivity

0.338 ng/mL

Assay Range

6.3 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human Vitamin D BP

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Vitamin D BP immunoassay is a 3.5 hour sandwich-type solid phase ELISA designed to measure human Vitamin D BP in cell culture supernates, serum, plasma, and urine. It contains HEK293-expressed recombinant human Vitamin D BP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Vitamin D BP showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Vitamin D BP.

Read more about the Vitamin D BP specificity in this White Paper.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	10.9	31.7	63.7	10.1	30.8	61.9
Standard Deviation	0.244	0.423	1.36	0.661	0.664	1.31
CV%	2.2	1.3	2.1	6.5	2.2	2.1

Recovery

The recovery of human Vitamin D BP spiked to three different levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	93-117
Urine (n=4)	89	78-96

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Vitamin D BP were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

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