Human TFPI Quantikine ELISA Kit

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Human TFPI Quantikine ELISA Kit

Human TFPI Quantikine ELISA Kit Summary

货号：DTFP10 规格：96T

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (50 uL)

Sensitivity

6.7 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)

Specificity

Natural and recombinant human TFPI. This kit recognizes predominantly free TFPI and a very small percentage of the LDL- and HDL-bound TFPI.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human TFPI Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human TFPI in cell culture supernates, plasma, and urine. It contains NS0-expressed recombinant human TFPI and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TFPI showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TFPI.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	299	554	1108	301	569	1127
Standard Deviation	13.5	22.2	40.3	18.5	30.6	66.2
CV%	4.5	4	3.6	6.1	5.4	5.9

Recovery

The recovery of TFPI spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	92	85-100
Urine (n=4)	94	85-106

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of TFPI were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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