Human Fas Ligand/TNFSF6 Quantikine ELISA

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Human Fas Ligand/TNFSF6 Quantikine ELISA Kit

Human Fas Ligand/TNFSF6 Quantikine ELISA Kit Summary

货号：DFL00B 规格：96T

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Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

5.1 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Fas Ligand.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Fas Ligand/TNFSF6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Fas Ligand in cell culture supernates, cell lysates, serum, and plasma. It contains CHO cell-expressed recombinant human Fas Ligand and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Fas Ligand showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Fas Ligand.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	92.1	240	587	98.6	244	576
Standard Deviation	3.31	7.51	18.2	4.41	8.7	25.7
CV%	3.6	3.1	3.1	4.5	3.6	4.5

Recovery

The recovery of human Fas Ligand spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	98	93-103
EDTA Plasma (n=4)	103	94-111
Heparin Plasma (n=4)	96	92-101
Serum (n=4)	101	96-103

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