Human Total MMP-7 Quantikine ELISA Kit

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Human Total MMP-7 Quantikine ELISA Kit

Human Total MMP-7 Quantikine ELISA Kit Summary

Catalog Size / Price

DMP700 1 Kit / Please Inquire
SMP700 1 Pack (6 Plates) / Please Inquire

PDMP700 1 Pack (50 Plates) / Please Inquire

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 uL), Serum (25 uL), Heparin Plasma (25 uL), Saliva (25 uL), Urine (50 uL)

Sensitivity

0.094 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)

Specificity

Natural and recombinant human MMP-7

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MMP-7 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MMP-7 in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human MMP-7 and antibodies raised against the recombinant factor. The Quantikine kit detected both pro- and active forms of recombinant human MMP-7. Natural human MMP-7 showed dose-response curves that were parallel to the standard curves obtained using the kit standards, indicating that this kit can be used to determine relative levels of natural human MMP-7.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	1.26	4.58	6.29	1.23	4.82	6.76
Standard Deviation	0.064	0.168	0.217	0.054	0.198	0.309
CV%	5.1	3.7	3.4	4.4	4.1	4.6

Recovery

The recovery of MMP-7 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	96-108
Urine (n=2)	99	89-109

Linearity

To assess the linearity of the assay, samples spiked with or containing high concentrations of MMP-7 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line

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