Human Pentraxin 3/TSG-14 Quantikine ELIS

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Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

Human Pentraxin 3/TSG-14 Quantikine ELISA Kit Summary

货号：DPTX30

规格：96T

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

5 hours

Sample Type & Volume Required Per Well

Serum-free Cell Culture Supernates (100 uL), EDTA Plasma (100 uL), Saliva (100 uL)

Sensitivity

0.116 ng/mL

Assay Range

0.3 - 20 ng/mL (Serum-free Cell Culture Supern, EDTA Plasma, Saliva)

Specificity

Natural and recombinant human PTX3

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human PTX3 Immunoassay is a 5.0 hour solid-phase ELISA designed to measure human PTX3 in pretreated cell culture supernates, plasma, and saliva. It contains NS0-expressed recombinant human PTX3 and has been shown to accurately quantitate the recombinant factor. Results obtained using pretreated natural human PTX3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PTX3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum-free Cell Culture Supernates, EDTA Plasma, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	2.61	7.72	14.1	2.75	7.94	14.5
Standard Deviation	0.01	0.28	0.62	0.17	0.39	0.6
CV%	3.8	3.7	4.4	6.1	5	4.1

Recovery

The recovery of PTX3 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	99	83-106
Serum-free Cell Culture Media (n=4)	101	95-108

Linearity

To assess the linearity of the assay, pretreated samples containing and/or spiked with high concentrations of PTX3 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

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