Human MIF Quantikine ELISA Kit

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Human MIF Quantikine ELISA Kit

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Human MIF Quantikine ELISA Kit Summary

Catalog Availability
DMF00B 1 Kit / Please Inquire
SMF00B 1 Pack (6 Plates) / Please Inquire

PDMF00B 1 Pack (50 Plates) / Please Inquire

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL), Urine (10 uL), Human Milk (10 uL)

Sensitivity

0.068 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)

Specificity

Natural and recombinant human MIF

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure human MIF in cell culture supernates, serum, plasma, urine, and human milk. It contains E. coli-expressed recombinant human MIF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human MIF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	1.16	3.56	7.27	1.03	3.2	6.46
Standard Deviation	0.07	0.18	0.33	0.1	0.27	0.61
CV%	6	5.1	4.5	9.7	8.4	9.4

Recovery

The recovery of MIF spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	95	86-104
Platelet-poor EDTA Plasma (n=5)	95	80-108
Platelet-poor Heparin Plasma (n=5)	96	86-108
Serum (n=4)	99	94-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MIF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line

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