Human CCL22/MDC Quantikine ELISA Kit

返回产品中心

Human CCL22/MDC Quantikine ELISA Kit

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

Human CCL22/MDC Quantikine ELISA Kit Summary

货号：DMD00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)

Sensitivity

62.5 pg/mL

Assay Range

125.0 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human MDC

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MDC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human MDC in cell culture supernates, serum, or plasma. It contains E. coli-expressed recombinant human MDC and has been shown to accurately quantitate the recombinant factor. Results obtained using naturally expressed human MDC showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed human MDC.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	284	452	821	276	440	810
Standard Deviation	12	9.7	24	22.2	27.8	42.8
CV%	4.2	2.1	2.9	8	6.3	5.3

Recovery

The recovery of MDC spiked to three different levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	90-110

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of MDC were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line

Human SCF Quantikine ELISA Kit