Human TIMP-2 Quantikine ELISA Kit

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Human TIMP-2 Quantikine ELISA Kit

Human TIMP-2 Quantikine ELISA Kit Summary

货号：DTM200

规格：96T

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL)

Sensitivity

0.064 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)

Specificity

Natural and recombinant human TIMP-2. This assay detects approximately 50% of recombinant human TIMP-2 when complexed with recombinant human active MMP-9 in a 1:1 molar ratio.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human TIMP-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIMP-2 levels in cell culture supernates, serum, plasma, and saliva. It contains CHO cell-expressed recombinant human TIMP-2, and antibodies raised against the recombinant protein. Natural human TIMP-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIMP-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	1.23	3.45	6.09	1.26	3.45	6.38
Standard Deviation	0.054	0.116	0.181	0.086	0.197	0.467
CV%	4.4	3.4	3	6.8	5.7	7.3

Cell Culture Supernates, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	1.01	2.9	5.26	1.01	2.79	5.27
Standard Deviation	0.042	0.173	0.344	0.079	0.188	0.326
CV%	4.2	6	6.5	7.8	6.7	6.2

Recovery

The recovery of TIMP-2 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	95	81-108

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of TIMP-2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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