Human IL-13 Quantikine ELISA Kit

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Human IL-13 Quantikine ELISA Kit

Human IL-13 Quantikine ELISA Kit Summary

Catalog Size / Price
D1300B 1Kit / Please Inquire
S1300B 1 Pack (6 Plates) / Please Inquire

PD1300B 1 Pack (50 Plates) / Please Inquire

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)

Sensitivity

57.4 pg/mL

Assay Range

125.0 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IL-13

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human IL-13 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-13 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-13 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-13 accurately. Results obtained using natural human IL-13 showed dose response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-13.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	352	960	1919	365	1087	2199
Standard Deviation	11.1	21.3	33.3	25.8	63.1	105
CV%	3.2	2.2	1.7	7.1	5.8	4.8

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	360	1031	2002	319	932	1895
Standard Deviation	12.9	27.5	43.2	22.2	52.6	108
CV%	3.6	2.7	2.2	7	5.6	5.7

Recovery

The recovery of IL-13 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	108	100-120
EDTA Plasma (n=4)	106	98-120
Heparin Plasma (n=4)	104	94-120
Serum (n=4)	102	95-113

Linearity

To assess linearity of the assay, samples were spiked with high concentrations of IL-13 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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