Human CXCL5/ENA-78 Quantikine ELISA Kit

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Human CXCL5/ENA-78 Quantikine ELISA Kit

Human CXCL5/ENA-78 Quantikine ELISA Kit Summary

货号：DX000

规格: 96T

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL), Citrate Plasma (50 uL)

Sensitivity

15 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human ENA-78

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human ENA-78 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human ENA-78 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human ENA-78 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant human ENA-78 accurately. Results obtained using natural human ENA-78 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human ENA-78.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	113	244	995	109	240	989
Standard Deviation	9.4	12.9	37.8	10.1	17.8	65.8
CV%	8.3	5.3	3.8	9.3	7.4	6.7

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	112	251	1096	129	278	1085
Standard Deviation	6	14.6	44.9	12.6	26.8	96.5
CV%	5.4	5.8	4.1	9.8	9.6	8.9

Recovery

The recovery of ENA-78 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	101	95-107
Citrate Plasma (n=5)	100	94-106
EDTA Plasma (n=5)	98	92-104
Heparin Plasma (n=5)	98	93-103
Serum (n=5)	101	93-109

Linearity

To assess the linearity of the assay, five samples containing or spiked with high concentrations of ENA-78 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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