Human FGF basic Quantikine HS ELISA Kit

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Human FGF basic Quantikine HS ELISA Kit

Human FGF basic Quantikine HS ELISA Kit Summary

Catalog Size / Price

HSFB00D 1 Kit / Please Inquire
SSFB00D 1 Pack (6 Plates) / Please Inquire

PHSFB00D 1 Pack (50 Plates) / Please Inquire

Assay Length

5.5 hours

Sample Type & Volume Required Per Well

Serum (100 uL), EDTA Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)

Sensitivity

0.07 pg/mL

Assay Range

0.3 - 20 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)

Specificity

Natural and recombinant human FGF basic

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine HS Human FGF basic Immunoassay kit is a 5.5 hour solid phase ELISA designed to measure FGF basic levels in serum, plasma, and urine. It contains E. coli-expressed recombinant human FGF basic and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human FGF basic accurately. Results obtained using natural human FGF basic showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human FGF basic.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	1.31	5.32	11.4	1.51	5.71	11.1
Standard Deviation	0.1	0.23	0.4	0.12	0.27	0.91
CV%	7.7	4.4	3.5	8.2	4.7	8.2

Recovery

The recovery of FGF basic spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Citrate Plasma (n=4)	97	83-118
EDTA Plasma (n=4)	99	85-109
Serum (n=4)	100	88-116
Urine (n=4)	109	100-120

Linearity

To assess the linearity of the assay, the following biological samples spiked with high concentrations of FGF basic were diluted with Calibrator Diluent and assayed.

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