Mouse CXCL9/MIG Quantikine ELISA Kit

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Mouse CXCL9/MIG Quantikine ELISA Kit

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Mouse CXCL9/MIG Quantikine ELISA Kit Summary

货号:MCX900 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), EDTA Plasma (25 uL)

Sensitivity

7.8 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma)

Specificity

Natural and recombinant mouse MIG

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse MIG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MIG in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse MIG and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse MIG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MIG.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	23	23	23
Mean	77	216	815	66	190	741
Standard Deviation	6.2	14.8	68	5.5	14.1	39.5
CV%	8.1	6.9	8.3	8.3	7.4	5.3

Recovery

The recovery of mouse MIG spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	95	81-116
EDTA Plasma (n=9)	94	81-107
Serum (n=9)	93	85-112
Tissue Homogenates (n=2)	104	88-118

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MIG in each matrix were diluted with Calibrator Diluent and then assayed.

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