Human IL-4 Quantikine HS ELISA Kit

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Human IL-4 Quantikine HS ELISA Kit

Human IL-4 Quantikine HS ELISA Kit Summary

Catalog Size / Price
HS400 1 Kit / Please Inquire
SS400 1 Pack (6 Plates) / Please Inquire
PHS400 1 Pack (50 Plates) / Please Inquire

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

6.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (200 uL), Serum (200 uL)

Sensitivity

0.22 pg/mL

Assay Range

0.3 - 16 pg/mL (Cell Culture Supernates, Serum)

Specificity

Natural and recombinant human IL-4

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine HS Human IL-4 Immunoassay is a 6.5 hour solid phase ELISA designed to measure IL-4 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant human IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant human IL-4 accurately. Results obtained using natural human IL-4 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-4.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	1.75	5.56	10.7	0.59	3.32	10.7
Standard Deviation	0.05	0.28	0.29	0.06	0.19	0.79
CV%	2.9	5	2.7	10.2	5.7	7.4

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	0.68	3.79	12.3	0.61	3.56	11.1
Standard Deviation	0.05	0.3	0.94	0.06	0.29	0.9
CV%	7.4	7.9	7.6	9.8	8.1	8.1

Recovery

The recovery of IL-4 was determined by spiking to levels throughout the range of the assay in various matrices.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=9)	106	87-118
Serum (n=4)	102	95-108

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