Human Apolipoprotein B/ApoB Quantikine E

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Human Apolipoprotein B/ApoB Quantikine ELISA Kit

Human Apolipoprotein B/ApoB Quantikine ELISA Kit Summary

货号：DAPB00

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

9.97 ng/mL

Assay Range

39.1 - 2,500 ng/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural human ApoB

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Apolipoprotein B/ApoB Immunoassay is a 4.5 hour solid phase ELISA designed to measure ApoB levels in serum and plasma.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	284	654	1228	261	717	1375
Standard Deviation	15	33.6	48.1	27.4	61.2	90.5
CV%	5.3	5.1	3.9	10.5	8.5	6.6

Linearity

To assess the linearity of the assay, samples containing high concentrations of ApoB were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Product Summary

Precision

Linearity

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