Human Thrombospondin-2 Quantikine ELISA

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Human Thrombospondin-2 Quantikine ELISA Kit

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Human Thrombospondin-2 Quantikine ELISA Kit Summary

货号：DTSP20 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL), Human Milk (50 uL)

Sensitivity

0.068 ng/mL

Assay Range

0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk)

Specificity

Natural and recombinant human Thrombospondin-2

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Thrombospondin-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Thrombospondin-2 in cell culture supernates, serum, plasma, and human milk. It contains NS0-expressed recombinant human Thrombospondin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Thrombospondin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Thrombospondin-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	4.08	7.73	10.9	4.11	7.71	11.4
Standard Deviation	0.189	0.229	0.281	0.295	0.511	0.726
CV%	4.6	3	2.6	7.2	6.6	6.4

Serum, EDTA Plasma, Heparin Plasma, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	3.97	7.96	11.4	4.12	7.68	11.4
Standard Deviation	0.198	0.205	0.284	0.259	0.403	0.527
CV%	5	2.6	2.5	6.3	5.2	4.6

Recovery

The recovery of Thrombospondin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	91-108
EDTA Plasma (n=4)	93	86-105
Heparin Plasma (n=4)	92	86-103
Serum (n=4)	93	86-105

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Thrombospondin-2 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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