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Human Relaxin-2 Quantikine ELISA Kit

    

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货号：DRL200   规格：96T

Human Relaxin-2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
4.57 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Relaxin-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Relaxin-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Relaxin-2 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Relaxin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Relaxin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Relaxin-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 42 42 42
Mean 34.4 99.6 206 40.8 112 220
Standard Deviation 1.6 2.47 4.75 4.16 7.02 12.1
CV% 4.7 2.5 2.3 10.2 6.3 5.5

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 41 41 41
Mean 35.1 96 194 34.9 98.9 197
Standard Deviation 1.27 2.44 4.01 3.41 5.9 11.7
CV% 3.6 2.5 2.1 9.8 6 5.9

Recovery

The recovery of Relaxin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 89-102
EDTA Plasma (n=4) 98 93-106
Heparin Plasma (n=4) 97 91-104
Serum (n=4) 101 92-107

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