Human Leptin R Quantikine ELISA Kit

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Human Leptin R Quantikine ELISA Kit

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Human Leptin R Quantikine ELISA Kit Summary

货号：DOBR00 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.128 ng/mL

Assay Range

0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Leptin sR

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human Leptin sR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Leptin sR in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Leptin R/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Leptin sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Leptin sR.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	1.83	5.15	11.2	1.69	5.09	10.7
Standard Deviation	0.071	0.283	0.292	0.097	0.282	0.633
CV%	3.9	5.5	2.6	5.7	5.5	5.9

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	2.34	7.01	14.6	2.34	6.95	14.3
Standard Deviation	0.142	0.155	0.709	0.202	0.37	0.966
CV%	6.1	2.2	4.9	8.6	5.3	6.7

Recovery

The recovery of human Leptin sR spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	96	91-102
EDTA Plasma (n=4)	104	92-115
Heparin Plasma (n=4)	103	92-113
Serum (n=4)	106	95-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Leptin sR were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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