Human u-Plasminogen Activator/Urokinase

返回产品中心

Human u-Plasminogen Activator/Urokinase Quantikine ELISA

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

Human u-Plasminogen Activator/Urokinase Quantikine ELISA Summary

货号：DUPA00 规格:96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL), Urine (10 uL)

Sensitivity

4.17 pg/mL

Assay Range

31.3 - 2,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human uPA. This assay also recognizes uPA complexed with PAI-1 or uPAR.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human u-Plasminogen Activator/Urokinase Immunoassay is a 4.5 hour solid phase ELISA designed to measure uPA in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human uPA and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human uPA. Results obtained using natural human uPA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human uPA.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	230	629	1219	214	614	1188
Standard Deviation	4.8	8.9	29.3	15.1	39.8	82
CV%	2.1	1.4	2.4	7.1	6.5	6.9

Recovery

The recovery of uPA spiked to three different levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=8)	102	93-115
Cell Lysates (n=4)	97	81-116
EDTA Plasma (n=4)	87	83-94
Heparin Plasma (n=4)	89	81-110
Serum (n=4)	87	81-93
Urine (n=4)	98	81-119

Linearity

To assess the linearity of the assay, samples containing high concentrations of uPA were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Human Leptin R Quantikine ELISA Kit