Mouse/Rat PDGF-BB Quantikine ELISA Kit

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Mouse/Rat PDGF-BB Quantikine ELISA Kit

Mouse/Rat PDGF-BB Quantikine ELISA Kit Summary

货号：MBB00 规格：96T

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

19.3 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse and rat PDGF-BB. This assay cross-reacts 10.2% with recombinant Rat PDGF-AB, 5.3% with recombinant Human PDGF-AB, 64.7% with recombinant Human PDGF-BB, 38.1% with natural Human PDGF and 65.2% with natural Porcine PDGF.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse/Rat PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse/rat PDGF-BB in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat PDGF-BB and antibodies raised against recombinant human PDGF-BB. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse/rat PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat PDGF-BB.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	22	24	24
Mean	49.7	252	823	52.1	246	811
Standard Deviation	4.5	18.7	36.7	5.3	21.1	40.4
CV%	9.1	7.4	4.5	10.2	8.6	5

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	22	22	23
Mean	61.1	298	1019	69.5	305	1048
Standard Deviation	5.6	24.6	40.3	6.6	26.3	98.5
CV%	9.2	8.3	4	9.5	8.6	9.4

Recovery

The recovery of PDGF-BB spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Mouse Cell Culture Supernates (n=8)	100	89-118
Mouse Heparin Plasma (n=5)	96	85-113
Rat Cell Culture Supernates (n=8)	102	94-114
Rat EDTA Plasma (n=5)	102	86-111
Rat Heparin Plasma (n=5)	102	85-111
Rat Serum (n=5)	100	89-113

Linearity

To assess the linearity of the assay, samples spiked with or containing PDGF-BB in each matrix were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Mouse TSLP Quantikine ELISA Kit

Mouse/Rat HGF Quantikine ELISA Kit

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Product Summary

Precision

Recovery

Linearity

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