Mouse/Rat HGF Quantikine ELISA Kit

返回产品中心

Mouse/Rat HGF Quantikine ELISA Kit

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

Mouse/Rat HGF Quantikine ELISA Kit Summary

货号：MHG00 规格:96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Homogenates (25 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL)

Sensitivity

12.1 pg/mL

Assay Range

62.5 - 4,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma)

Specificity

Natural and recombinant HGF. Recombinant mouse HGF single chain precursor cross-reacts 100% in this assay.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat HGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat HGF in cell culture supernates, tissue homogenates, serum, and platelet-poor plasma. It contains NS0-expressed recombinant mouse HGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural HGF showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	217	452	1168	218	447	1107
Standard Deviation	8.6	19.7	62.2	14.9	21	87.7
CV%	4	4.4	5.3	6.8	4.7	7.9

Recovery

The recovery of HGF spiked into various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Mouse Cell Culture Supernates (n=4)	98	85-106
Mouse Platelet-poor EDTA Plasma (n=4)	104	101-106
Mouse Serum (n=4)	96	84-112
Mouse Tissue Homogenates (n=3)	87	80-101
Rat Cell Culture Supernates (n=4)	102	85-114
Rat Platelet-poor EDTA Plasma (n=4)	108	100-116

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse/rat HGF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Mouse/Rat PDGF-BB Quantikine ELISA Kit