Human Pro-IL-1 beta/IL-1F2 Quantikine EL

返回产品中心

Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit

Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary

货号：DLBP00 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL)

Sensitivity

8.9 pg/mL

Assay Range

23.4 - 1,500 pg/mL (Cell Culture Supernates), 31.2 - 2,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human pro-IL-1 beta

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Pro-IL-1 beta is a sandwich enzyme-linked immunoassay. The capture antibody coated to the microplate is specific for the pro-piece of the molecule (first 116 residues), and the detection antibody is specific for mature IL-1 beta. Thus, the assay does not detect either the pro-piece alone or the mature form alone; it is specific instead for the intact pro-IL-1 beta.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	85.5	240	432	85.1	248	457
Standard Deviation	4.7	11.5	20.8	6.2	14	28.3
CV%	5.5	4.8	4.8	7.3	5.6	6.2

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	126	374	670	121	342	626
Standard Deviation	6.4	15.7	34.5	8.8	17.9	34.9
CV%	5.1	4.2	5.1	7.3	5.2	5.6

Recovery

The recovery of pro-IL-1 beta spiked to three different levels throughout the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	108	99-115
EDTA Plasma (n=5)	103	95-108
Heparin Plasma (n=5)	101	92-109
Serum (n=5)	102	97-108

Linearity

To assess the linearity of the assay, five samples spiked with high concentrations of pro-IL-1 beta were diluted in the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Kallikrein 3/PSA Quantikine ELISA Kit

Human LIF Quantikine ELISA Kit

返回 产品中心

Product Summary

Precision

Recovery

Linearity

返回产品中心