Human Kallikrein 3/PSA Quantikine ELISA

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Human Kallikrein 3/PSA Quantikine ELISA Kit

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Human Kallikrein 3/PSA Quantikine ELISA Kit Summary

货号：DKK300 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

0.069 ng/mL

Assay Range

0.9 - 60 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human KLK3/PSA

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human KLK3/PSA Immunoassay is a 4.5 hour solid phase immunoassay designed to measure KLK3/PSA in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human KLK3/PSA, and antibodies raised against the recombinant protein. Natural human KLK3/PSA showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human KLK3/PSA.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	12.3	24.3	44.1	11.8	24.2	43.8
Standard Deviation	0.7	1.3	2	0.9	0.5	0.8
CV%	5.7	5.4	4.6	7.7	5.8	5.5

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	6.96	15.5	30	7.38	16.7	33
Standard Deviation	0.5	0.7	0.9	0.5	0.9	1.6
CV%	7.8	4.5	3	6.4	5.3	5

Recovery

The recovery of KLK3/PSA spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	99	93-105
EDTA Plasma (n=4)	100	93-103
Heparin Plasma (n=4)	101	97-109
Serum (n=4)	102	97-109

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of KLK3/PSA were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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