Human SP-D Quantikine ELISA Kit

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Human SP-D Quantikine ELISA Kit

Human SP-D Quantikine ELISA Kit Summary

货号：DSFPD0

规格：96T

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

5.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

0.37 ng/mL

Assay Range

0.6 - 40 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human SP-D

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human SP-D Immunoassay is a 5.5 hour solid-phase ELISA designed to measure human SP-D in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human SP-D and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SP-D showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally ocurring SP-D.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	6.07	11.7	23.4	5.63	11.2	22.8
Standard Deviation	0.495	0.588	1.46	0.495	0.999	1.78
CV%	8.2	5	6.2	8.8	8.9	7.8

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	5.99	11.3	24.3	6.63	13.1	26.9
Standard Deviation	0.371	0.921	1.63	0.617	1.14	2.5
CV%	6.2	8.2	6.7	9.3	8.7	9.3

Recovery

The recovery of SP-D spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	95-109
EDTA Plasma (n=4)	110	99-120
Heparin Plasma (n=4)	104	85-118
Serum (n=4)	106	89-118

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SP-D were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human LIF Quantikine ELISA Kit

Human Total MMP-8 Quantikine ELISA Kit

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Product Summary

Precision

Recovery

Linearity

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