Human Total MMP-8 Quantikine ELISA Kit

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Human Total MMP-8 Quantikine ELISA Kit

Human Total MMP-8 Quantikine ELISA Kit Summary

货号：DMP800B

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Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (25 uL), Platelet-poor EDTA Plasma (50 uL), Heparin Plasma (25 uL), Platelet-poor Heparin Plasma (50 uL), Saliva (10 uL)

Sensitivity

0.058 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva)

Specificity

Natural and recombinant human MMP-8.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Total MMP-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure total MMP-8 (pro- and active MMP-8) in cell culture supernates, tissue lysates, serum, plasma, platelet-poor plasma, and saliva. It contains NS0-expressed recombinant human MMP-8, and antibodies raised against the recombinant protein. Natural human MMP-8 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MMP-8.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	0.764	2.38	4.55	0.836	2.41	5.07
Standard Deviation	0.049	0.07	0.299	0.055	0.171	0.27
CV%	6.4	2.9	6.6	6.6	7.1	5.3

Recovery

The recovery of human Total MMP-8 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	93-103
EDTA Plasma (n=4)	92	86-99
Extraction Buffer (1X) (n=2)	84	83-85
Heparin Plasma (n=4)	92	86-99
Platelet-poor EDTA Plasma (n=4)	85	79-91
Platelet-poor Heparin Plasma (n=4)	84	78-89
Saliva (n=4)	96	86-111
Serum (n=4)	101	93-109

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Total MMP-8 in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

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Product Summary

Precision

Recovery

Linearity

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