Human IL-18 BPa Quantikine ELISA Kit

返回产品中心

Human IL-18 BPa Quantikine ELISA Kit

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

Human IL-18 BPa Quantikine ELISA Kit Summary

货号：DBP180

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)

Sensitivity

7.52 pg/mL

Assay Range

26.6 - 1,700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human IL-18 BPa

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human IL-18 BPa Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-18 BPa in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human IL-18 BPa and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-18 BPa. Results obtained using natural human IL-18 BPa showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18 BPa.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	109	368	740	115	362	692
Standard Deviation	5.73	16.4	15.8	13.5	29.1	48.3
CV%	5.3	4.5	2.1	11.7	8	7

Cell Culture Supernates, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	123	404	789	136	434	841
Standard Deviation	5.5	9.49	21.1	9.47	32.8	59.1
CV%	4.5	2.3	2.7	7	7.6	7

Recovery

The recovery of human IL-18 BPa spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	106	98-114
EDTA Plasma (n=4)	106	93-113
Heparin Plasma (n=4)	105	92-119
Serum (n=4)	102	92-113

Linearity

To assess the linearity of the assay, samples containing high concentrations of human IL-18 BPa were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human IGF-II Quantikine ELISA Kit