Human Galectin-9 Quantikine ELISA Kit

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Human Galectin-9 Quantikine ELISA Kit

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Human Galectin-9 Quantikine ELISA Kit Summary

货号：DGAL90

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Saliva (100 uL), Urine (10 uL), Human Milk (10 uL)

Sensitivity

0.028 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

Specificity

Natural and recombinant human Galectin-9.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Galectin-9 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Galectin-9 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Galectin-9 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Galectin-9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Galectin-9.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Saliva, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	1.34	3.58	6.13	1.44	3.43	6.05
Standard Deviation	0.05	0.12	0.22	0.08	0.18	0.32
CV%	3.7	3.4	3.6	5.6	5.2	5.3

Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	1.44	3.77	6.29	1.4	3.28	5.84
Standard Deviation	0.06	0.07	0.08	0.11	0.2	0.3
CV%	4.2	1.9	1.3	7.9	6.1	5.1

Recovery

The recovery of human Galectin-9 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	96	84-104
EDTA Plasma (n=4)	103	85-117
Heparin Plasma (n=4)	104	89-117
Serum (n=4)	106	86-117

Linearity

To assess the linearity of the assay, samples containing high concentrations of human Galectin-9 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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