Human Fas/TNFRSF6/CD95 Quantikine ELISA

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Human Fas/TNFRSF6/CD95 Quantikine ELISA Kit

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Human Fas/TNFRSF6/CD95 Quantikine ELISA Kit Summary

货号：DFS00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)

Sensitivity

20 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human sFas

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human sFas Immunoassay is a 4.5 hour solid phase ELISA designed to measure human sFas in cell culture supernates, serum, and plasma. It contains recombinant human Fas/Fc chimera expressed from NS0 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human sFas showed dose-response curves that were parallel to the standard curves obtained using the recombinant Fas/Fc chimera. These results indicate that this kit can be used to determine relative mass values for natural human sFas.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	130	804	1424	140	811	1391
Standard Deviation	5	23.3	65.2	9.4	31.8	40
CV%	3.8	2.9	4.6	6.7	3.9	2.9

Recovery

The recovery of Fas/Fc chimera spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	92-107
Citrate Plasma (n=5)	98	82-116
EDTA Plasma (n=5)	97	86-111
Heparin Plasma (n=5)	94	88-109
Serum (n=5)	97	91-110

Linearity

To assess the linearity of the assay, five samples containing or spiked with high concentrations of sFas or Fas/Fc chimera were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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