Human GM-CSF Quantikine HS ELISA Kit

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Human GM-CSF Quantikine HS ELISA Kit

Human GM-CSF Quantikine HS ELISA Kit Summary

货号：HSGM0

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

6.5 hours

Sample Type & Volume Required Per Well

Serum (150 uL), EDTA Plasma (150 uL), Heparin Plasma (150 uL), Urine (200 uL)

Sensitivity

0.26 pg/mL

Assay Range

1.0 - 64 pg/mL (Serum, EDTA Plasma, Heparin Plasma), 0.5 - 32 pg/mL (Urine)

Specificity

Natural and recombinant human GM-CSF

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine HS Human GM-CSF Immunoassay is a 6.5 hour solid phase ELISA designed to measure human GM-CSF in serum, plasma, and urine. It contains E. coli-expressed recombinant human GM-CSF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural GM-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human GM-CSF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	2.69	7.05	24.8	3.77	14.3	27.4
Standard Deviation	0.14	0.33	0.73	0.37	1.17	1.85
CV%	5.2	4.7	2.9	9.8	8.2	6.8

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	18	18	18
Mean	3.68	22	44.6	2.8	16.2	33.2
Standard Deviation	0.35	1.07	1.76	0.66	2.47	4.6
CV%	9.5	4.9	4	23.5	15.3	13.9

Recovery

The recovery of GM-CSF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	114	88-140
Heparin Plasma (n=4)	109	91-122
Serum (n=4)	109	94-128
Urine (n=5)	102	95-116

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of GM-CSF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Product Summary

Precision

Recovery

Linearity

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