Mouse Resistin Quantikine ELISA Kit

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Mouse Resistin Quantikine ELISA Kit

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Mouse Resistin Quantikine ELISA Kit Summary

货号：MRSN00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

8 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse Resistin

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse Resistin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Resistin in cell culture supernates, mouse serum, and plasma. It contains E. coli-expressed recombinant mouse Resistin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Resistin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse Resistin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	41	41	41
Mean	84	245	494	84	239	508
Standard Deviation	3.4	7.2	13.3	6.1	16.4	34.9
CV%	4	2.9	2.7	7.3	6.9	6.9

Recovery

The recovery of mouse Resistin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=8)	99	85-108
EDTA Plasma (n=8)	97	87-107
Heparin Plasma (n=8)	102	89-113
Serum (n=6)	99	93-108

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse Resistin in each matrix were diluted with Calibrator Diluent and then assayed.

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