Human DPPIV/CD26 Quantikine ELISA Kit

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Human DPPIV/CD26 Quantikine ELISA Kit

Human DPPIV/CD26 Quantikine ELISA Kit Summary

货号：DC260B

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Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (50 uL)

Sensitivity

0.072 ng/mL

Assay Range

0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)

Specificity

Natural and recombinant human DPPIV.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human DPPIV/CD26 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DPPIV in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human DPPIV, and antibodies raised against the recombinant factor. Results obtained using natural human DPPIV showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human DPPIV.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Saliva, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	2.16	8.15	14.1	2.22	8.25	14.6
Standard Deviation	0.105	0.28	0.916	0.14	0.695	1.18
CV%	4.9	3.4	6.5	6.3	8.4	8.1

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	2.32	8.78	15.8	2.43	8.53	15.9
Standard Deviation	0.142	0.354	1.15	0.207	0.777	1.3
CV%	6.1	4	7.3	8.5	9.1	8.2

Recovery

The recovery of human DPPIV spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	87-113
Urine (n=4)	101	88-113

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human DPPIV were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

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Product Summary

Precision

Recovery

Linearity

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