Mouse VEGF R2/Flk-1 Quantikine ELISA Kit

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Mouse VEGF R2/Flk-1 Quantikine ELISA Kit

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Mouse VEGF R2/Flk-1 Quantikine ELISA Kit Summary

货号：MVR200B

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

0.049 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse sVEGF R2

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse soluble VEGF R2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R2 in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse sVEGF R2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	24	24	24
Mean	0.63	1.4	3.83	0.57	1.28	3.5
Standard Deviation	0.04	0.07	0.21	0.06	0.1	0.21
CV%	6.3	5	5.5	10.5	7.8	6

Recovery

The recovery of mouse sVEGF R2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	105	100-111
Plasma (n=4)	108	89-116
Serum (n=3)	104	82-120

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse sVEGF R2 in each matrix were diluted with Calibrator Diluent and then assayed.

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