Rat CXCL2/CINC-3 Quantikine ELISA Kit

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Rat CXCL2/CINC-3 Quantikine ELISA Kit

Rat CXCL2/CINC-3 Quantikine ELISA Kit Summary

货号：RCN300

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

2.7 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant rat CINC-3

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Rat CINC-3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure rat CINC-3 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat CINC-3 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat CINC-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat CINC-3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	50	46	50
Mean	111	345	1012	105	308	877
Standard Deviation	3.9	11.9	39.1	10.1	28.5	73.2
CV%	3.5	3.4	3.9	9.6	9.3	8.3

Recovery

The recovery of rat CINC-3 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	97	86-107
EDTA Plasma (n=6)	92	86-99
Heparin Plasma (n=6)	99	85-105
Serum (n=6)	97	83-106

Linearity

To assess the linearity of the assay, samples containing rat CINC-3 in each matrix were diluted with Calibrator Diluent and then assayed.

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Product Summary

Precision

Recovery

Linearity

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